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KMID : 0366220150500010046
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Korean Journal of Hematology
2015 Volume.50 No. 1 p.46 ~ p.50
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Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2
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Khosa Shafi Mohammad
Usman Muhammad
Moinuddin Moinuddin
Mehmood Hassan Osman
Qamar Khansa
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Abstract
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Background: The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels.
Methods: The test population comprised 160 individuals divided into four groups: normal individuals,β-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared.
Results: HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden’s index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively.
Conclusion: This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in ?-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective.
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KEYWORD
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Cellulose acetate hemoglobin electrophoresis, Hemoglobin A2, High performance liquid chromatography
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